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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be explained. Two further splice variants were described but in depth nucleotide sequence information is just not however out there.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly includes the epitope regarded with the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters check here to make sure the best possible results are...
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to be sure the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope to the focus on protein. By evaluating the staining from your blocked antibody compared to the antibody on your own, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is also degraded as cells development as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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